What is Superdex 200?

What is Superdex 200?

Superdex Increase 200 is a column for high resolution gel filtration in small scale (mg) preparative purification and also for characterization and analysis of proteins with molecular weights between 10,000 and 600,000, such as aggregate analysis of antibodies, size-homegeneity analysis of membrane proteins, or protein …

What is the void volume?

Void volume refers specifically to the volume of the liquid phase contained inside a column. The same term is sometimes also used informally to refer to the volume of a cavity in the column/tubing or fittings. Void volume is also known as dead volume.

What is Superdex 200 increase 10/300 GL?

Superdex 200 Increase 10/300 GL is suitable for small scale preparative purification (μg mg) as a final polishing step, as well as for protein analysis and characterization. The columns are supplied with two fingertight connectors 1

What is a Superdex 200 column?

Superdex 200 are prepacked size exclusion chromatography columns for high-resolution small-scale preparative and analytical separations of biomolecules. Superdex 200 has a separation range for molecules with molecular weights between 10 000 and 600 000.

What is cytiva LifeScience™ Superdex 200 increase 10/300 GL column used for?

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification. Need it Now?

What is the maximum concentration of protein in Superdex?

Molecular weight, M r 3 000–70 000 (Superdex 75) maximum 10 000–600 000 (Superdex 200) Protein concentration ≤ 10 mg in sample Sample volume of organic solvent25–500 µl Preparation (2,3)Dissolve the sample in eluent, in seriesfilter through a 0.22 µm filter or 10/300 GL and 2.5 MPa for Superdex 200 10/300 GL centrifuge at 10 000 g for 10 min.