What does the enolase enzyme do?

What does the enolase enzyme do?

Enolase is a glycolytic enzyme, which catalyzes the inter-conversion of 2-phosphoglycerate to phosphoenolpyruvate. Altered expression of this enzyme is frequently observed in cancer and accounts for the Warburg effect, an adaptive response of tumor cells to hypoxia.

What is the enolase reaction?

Enolase is an enzyme that catalyzes a reaction of glycolysis. Glycolysis converts glucose into two 3-carbon molecules called pyruvate. Enolase is used to convert 2-phosphoglycerate (2PG) to phosphoenolpyruvate (PEP) in the 9th reaction of glycolysis: it is a reversible dehydration reaction..

What type of catalysis is enolase?

Enolase is a dimeric enzyme that catalyzes the interconversion of 2-phospho-D-glycerate and phosphoenolpyruvate. This reversible dehydration is effected by general acid-base catalysis that involves, principally, Lys345 and Glu211 (numbering system of enolase 1 from yeast).

What does enolase do in gluconeogenesis?

Enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during both glycolysis and gluconeogenesis, and is required by all three domains of life.

What is the product of enolase?

Enolase (phosphopyruvate hydratase) is a metalloenzyme. It is responsible for the catalysis of the conversion of 2-phosphoglycerate (2-PG) to phosphoenolpyruvate (PEP), which is the ninth step of glycolysis.

What is the inhibitor of enolase enzyme?

Enolase is a dimeric enzyme that catalyzes the penultimate step in glycolysis, interconverting 2-phosphoglycerate (2-PGA) and phosphoenolpyruvate (PEP). The most potent enolase inhibitor described in the literature is Phosphoacetohydroxamate (PhAH, Fig.

What class of enzyme is enolase belong to?

Enolase belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme is 2-phospho-D-glycerate hydro-lyase (phosphoenolpyruvate-forming).

What is the structure of enolase?

Enolase consists of two identical subunits of 431 residues each. The primary structure of enolase is divided into two domains. The N-terminal domain consists of the first 133 residues and contains an antiparallel β-sheet of three strands and four alpha helices (Hosaka, et al., 820-821).

What happens when enolase is inhibited?

In addition to its significance for the brewing industry, the fluoride inhibition of enolase has practical ramifications in the treatment of dental plaque in which deep layers are highly anaerobic and dependent on glycolysis for energy requirements and, even more importantly, on the presence of PEP for the transport of …

What would be the EC number of enolase?

Enolase (EC 4.2. 1.11) is a cytosolic metalloenzyme responsible for the conversion of 2-phosphoglycerate into phosphoenolpyruvate, the second to last step in glycolysis.

How is enolase synthesized?

The reaction is reversible, depending on environmental concentrations of substrates. The optimum pH for the human enzyme is 6.5. Enolase is present in all tissues and organisms capable of glycolysis or fermentation….Enolase.

phosphopyruvate hydratase
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / QuickGO

What is the inhibitor of enolase?

What is neuron specific enolase?

Neuron-specific enolase. Neuron-specific enolase (NSE) is one of five isomers of the glycolytic enzyme enolase. It is localized within central and peripheral neurons as well as in neuroendocrine cells, but not in glial cells. Like the 14-3-3 proteins, this enzyme can be released into the CSF when neurons are injured.

What are the two types of enolase?

ββ or muscle-specific enolase (MSE). Also known as enolase 3. This enzyme is largely restricted to muscle where it is present at very high levels in muscle. γγ or neuron-specific enolase (NSE). Also known as enolase 2.

What is neneuron-specific enolase (NSE)?

Neuron-specific enolase (NSE) is known to be a cell specific isoenzyme of the glycolytic enzyme enolase. In vertebrate organisms three isozymes of enolase, expressed by different genes, are present: enolase α is ubiquitous; enolase β is muscle-specific and enolase γ is neuron-specific. The expressio …

What does enolase do in the human body?

Enolase, also known as phosphopyruvate hydratase, is a metalloenzyme responsible for the catalysis of the conversion of 2-phosphoglycerate (2-PG) to phosphoenolpyruvate (PEP), the ninth and penultimate step of glycolysis.