What color is Cy3?

orange
Cy3 dye dashboard The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.

Is Cy3 red or green?

Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC).

Is cy2 green?

Cyanine, Cy™2 Technical Information Cy™2 – conjugates have maximum absorption/excitation at 492 nm and fluoresce with a peak around 510 nm in the green region of the visible spectrum like FITC conjugates (520 nm), but they are more photostable and less sensitive to pH changes than FITC.

What wavelength is Tritc?

532 nm
Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.

Is Alexa 546 red?

Invitrogen Alexa Fluor 546 dye is a bright, orange-fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 546 dye is pH-insensitive over a wide molar range….Alexa Fluor 546 reactive dyes.

Amine	Thiol
NHS ester	Maleimide

What color is af488?

Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line.

What is FITC and TRITC?

FITC and TRITC are among the most simple and commonly used reagents for protein fluorescent labeling. These isothiocyanates will crosslink to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups on a protein. Excess nonreacted and hydrolyzed reagent can be removed by dialysis or gel filtration.

What does TRITC stand for?

TRITC

Acronym	Definition
TRITC	Tetramethyl Rhodamine Iso-Thiocyanate

Is Cy5 green or red?

Cy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.

Is af488 same as FITC?

Alexa Fluor 488 (Ex-Max 495 nm/Em-Max 519 nm): have nearly identical emission and excitation maxima as FITC. However, Alexa Flour 488 display higher photostability and is less susceptible to self-quenching.

What is Cy3 dye?

The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.

What is the best fluorescent dye for immunofluorescence?

Broadly used fluorescent dyes for immunofluorescence are FITC, TRITC or several Alexa Fluor ® dyes which are mentioned in the following. Fluorescein isothiocyanate ( FITC) is an organic fluorescent dye which is still used in immunofluorescence and flowcytometry.

What is Cy7 fluoride?

Cy7 is a near-IR fluor that is invisible to the naked eye (excitation/emission maximum 750/776 nm). It is used in in vivo imaging applications, as well as the Cy7.5 dye. Sulfo –Cyanine dyes bear one or two sulfo groups, rendering the Cy dye water-soluble, but tri- and quadri-sulfonated forms are available for even higher water solubility.

What is the fluorescence of a fluorescent dye?

FITC (389 g/mol) was one of the first dyes which was used for fluorescence microscopy and served as an origin for further fluorescent dyes like Alexa Fluor®488. Its fluorescence activity is due to its large conjugated aromatic electron system, which is excited by light in the blue spectrum.