Why do we use black plates for fluorescence?

For fluorescent assays, black plates absorb light and reduce background and crosstalk. Using a black plate in a multiplex assay will reduce fluorescent signal crosstalk and background, but will reduce the luminescence signal (you can expect to lose about 1 order of magnitude in luminescent signal using a black plate).

Can I use black plate for absorbance?

A good solid bright white plate is best for these absorbance measurements and a solid black plate for fluorescence readings. Bottom reading units illuminate the sample with monochromatic light from above and then use detectors placed below the plate to measure the absorption or fluorescence/luminescence emission.

Can I use black plates for luminescence?

White plates are used for luminescence and black plates are used for fluorescence. Plates with clear bottoms are used for bottom reading, and plates with white or black bottoms are used for top reading. Tissue culture-treated cells should be used for adherent cells.

Can you use clear plates for fluorescence?

Clear-bottom, TC-treated IsoPlate microplates could also be used for cell-based fluorescent assays. Clear-bottom IsoPlate microplates are similar to ViewPlate microplates in that the bottom of the plate is clear, while the sides of each well are either black or white.

What are black plates used for?

White plates reflect light and will maximize light output signal; black plates absorb light and reduce background and crosstalk. For these reasons, white plates are commonly used for luminescent assays and black plates are used for fluorescent assays.

How fluorescence is measured in a fluorescence plate reader?

Fluorescence plate readers are usually intended for fluorescence intensity and FRET detection. Consequently, fluorescence plate readers measure the light signal emitted by a sample in Relative Fluorescent Units (RFU).

Is a plate reader a spectrophotometer?

Microplate Readers, or Plate Readers, are a type of spectrophotometer. Both use either a monochromator system or filter-based optics and these lab instruments both collect useful measurements across a variety of methods.

Can you use TC treated plates for Elisa?

Tissue Culture-treated Polystyrene Non-specific background tends to be difficult to control. We advise that surfaces specified as Tissue Culture-treated not be used for ELISA or similar procedures.

What are 96 well plates used for?

Industry standard, 96-well plates are designed for all common instruments and can be used for applications such as sample collection, compound preparation, combinatorial chemistry, high throughput screening, nucleic acid purification, bacterial culture growth, and plate replication.

What is the purpose of using black plates instead of transparent plates?

Black pigmented plates are optimized for fluorescence measurements. The plates show a minimal auto-fluorescence because of the black pigmentation, especially in the lower wavelengths, as well as minimized crosstalk between the wells. Black plates are available as standard or with transparent bottom.

What are fluorescence-based assays?

Based on the types of fluorescent signals to be collected, fluorescence-based assays encompass diverse techniques that either macroscopically record the total fluorescence intensity, fluorescence polarization, fluorescence resonance energy transfer, time-resolved fluorescence, etc. or microscopically detect …

What does fluorescence spectroscopy measure?

Fluorescence spectroscopy analyzes fluorescence from a molecule based on its fluorescent properties. Fluorescence is a type of luminescence caused by photons exciting a molecule, raising it to an electronic excited state.

Are black or white plates better for fluorescence assays?

However, black plates may help in situations where cross-talk is an issue, resulting in better sensitivity. In general, we recommend white plates for higher-density time-resolved fluorescence assays (for example, assays run in 384-well and 1536-well plates) and other assays where a lower signal might be expected.

What type of microplate should I use for Lance ultra assays?

PerkinElmer recommends white OptiPlate ™ microplates for LANCE Ultra assays, both LANCE Ultra cAMP and LANCE Ultra kinase. Black microplates are generally used for prompt fluorescence assays to avoid the high autofluorescence generated by white plates.

What is a black and white microplate?

More Product Information Black and white microplates are manufactured from virgin polystyrene resin and are designed to meet industry standard dimension specifications to facilitate compatibility with microplate handling and robotics. LUMITRAC™ and FLUOTRAC™ formats are offered to allow a wide variety of applications, including cell-based assays.

What is a 96-well microplate used for?

Molecular Probes® 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and autofluorescence and so are optimized for fluorescence-based applications. These plates are compatible with standard fluorescence microplate readers and are offered in…