What is the confirmatory test for G6PD?

Your doctor can diagnose G6PD deficiency by performing a simple blood test to check G6PD enzyme levels. Other diagnostic tests that may be done include a complete blood count, serum hemoglobin test, and a reticulocyte count. All these tests give information about the red blood cells in the body.

Which lab test is most useful in diagnosing G6PD deficiency?

G6PD can be diagnosed with a quantitative spectrophotometric analysis or, more commonly, by a rapid fluorescent spot test. G6PD = glucose-6-phosphate dehydrogenase.

How does the G6PD fluorescent spot test work?

To perform the test, a small amount of blood is incubated with glucose-6-phosphate and NADP in the substrate reagent, and then is spotted on filter paper. Once dried, the spots are viewed under long-wave ultraviolet (UV) light—the by-product of the reaction (NADPH) is fluorescent.

Is G6PD part of newborn screening?

G6PD deficiency is a major contributor to neonatal hyperbilirubinemia, yet newborn screening for this disorder in the United States is not standard practice.

Why does the military test for G6PD?

When low G6PD levels impair the ability of erythrocytes to produce NADPH, those cells become susceptible to hemolysis. Recognizing this risk, the United States military routinely tests its personnel for G6PD deficiency before administering antimalarial drugs that can cause life-threatening hemolysis.

What is the normal reference range for G6PD?

5.5 to 20.5 units/
A normal test result tells your healthcare provider about G6PD activity in your blood cells. A normal—or no G6PDD—result for adults is 5.5 to 20.5 units/gram of hemoglobin. Less than 10% of normal means that you have severe deficiency and chronic hemolytic anemia.

What is Brewer’s test?

Methaemoglobin reduction test (Brewer’s test) is the most commonly used technique to screen the G6PD deficiency, which is the most common human enzyme deficiency in the world.

What does a high G6PD mean?

Levels of G6PD are higher in the newborn than they are in the adult. When high levels are seen in older patients, it invariably reflects the presence of a young red blood cell population with reticulocytosis.

What is a normal G6PD level?

The reference range for normal G6PD activity is 10.15–14.71 U/g Hb for neonates and 6.75–11.95 U/g Hb for adults.

What is the normal range for G6PD?

Can you join the Army with G6PD?

It is DoD policy that: a. All personnel entering the Military Services will be screened for G6PD deficiency.

Can you deploy with G6PD deficiency?

The U.S. military has therefore determined G6PD deficiency to be an important deployment health-and-readiness issue, and routinely conducts tests to avoid exposing G6PD-deficient individuals to antimalarial drugs that are more likely to cause hemolysis.

What is G6PD and how is it diagnosed?

What Is G6PD? Did I Inherit It? Screening means testing individuals to see if they have the deficiency. This is done by taking a sample of blood and then using one or more special tests to detect either a variant (mutation) in their DNA or a measurable deficiency in the activity of the enzyme in their red blood cells.

What is the Guthrie test?

The Guthrie test, also called the PKU test, is a diagnostic tool to test infants for phenylketonuria a few days after birth. To administer the Guthrie test, doctors use Guthrie cards to collect capillary blood from an infant’s heel, and the cards are saved for later testing.

Is the fluorescent spot test effective for screening for G6PD deficiency?

The World Health Organization has recommended that wherever the male population has an incidence of G6PD deficiency of greater than 3-5%, universal screening of newborns should be carried out using the fluorescent spot test.

What is the Guthrie test used to diagnose phenylketonuria?

By 1963 the test had become a routine neonatal test for phenylketonuria. The “Heel Prick” test. The Guthrie test or “Heel Prick” test is routinely carried out on neonatal (newborn 48-72 hours of age) blood for a variety of known genetic disorders.