What is TaqMan Copy Number assay?

TaqMan copy number assays are designed to detect and measure copy number variation in the human genome using real-time polymerase chain reaction and unquenching of fluorescent probes for the target sequence.

What is a reference assay?

KMRassay Reference Assay 901 contains the necessary genetic reference for the performance of KMRtype and KMRtrack. This assay targets a housekeeping gene outside the HLA region and has been designed to give a positive result for all human DNA.

What is RNase P assay?

The RNase P Assay (Cy5. 5) is a hydrolysis probe-based assay for the detection of RNase P. RNase P is a single-copy gene that is frequently used as a control. Use of the RNase P Assay can reduce false negative rates that can occur due to: Insufficient number of cells collected.

What is reference standard in HPLC?

HPLC standards are defined reference compounds or materials that test the quality, purity, and identity of a particular test sample by comparison. Ready-to-use HPLC standards can reduce cost, streamline production processes and ensure reproducibility in research and development.

What is standard reference?

A standard reference material (SRM) as defined by NIST “is a material or artifact that has had one or more of its property values certified by a technically valid procedure, and is accompanied by, or traceable to, a certificate or other documentation which is issued by NIST…

What is the role of ribonuclease P?

Ribonuclease P (RNase P) is an endoribonuclease that cleaves other RNA molecules at the junction between a single-stranded region and the 5′ end of a double-stranded region.

What is RNase P gene?

26.5, RNase P) is a type of ribonuclease which cleaves RNA. RNase P is unique from other RNases in that it is a ribozyme – a ribonucleic acid that acts as a catalyst in the same way that a protein-based enzyme would. Its function is to cleave off an extra, or precursor, sequence of RNA on tRNA molecules.

What is copy number biotechnology?

In cellular biology, the plasmid copy number is the number of copies of a given plasmid in a cell. To ensure survival and thus the continued propagation of the plasmid, they must regulate their copy number. Many biotechnology applications utilize mutated plasmids that replicate to high copy number.

How is copy number variation calculated?

The CNV is simply given by the ratio of the estimated concentration of the target gene to the estimated concentration of the reference gene. Both of these concentrations are estimated by applying the Poisson law as explained in the item Poisson Law Computation.

How do you identify copy number variations?

In NGS, millions of single end or mate pair reads are generated from the sample genomes with shotgun sequencing. CNVs are then detected based on the frequency of the reads (read depth) or aberrations in the mate pairs, after mapping the short reads to the reference genome.