What is T7 RNA polymerase used for?

T7 RNA Polymerase is used for in vitro mRNA synthesis and is highly specific for the T7 phage promoter. Applications include: Radiolabeled RNA probe preparation. RNA generation for studies of RNA structure, processing and catalysis.

What type of polymerase is T7?

T7 RNA Polymerase
T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA from DNA in the 5’→ 3′ direction.

What is E coli RNA polymerase?

E. coli RNA polymerase (EcoRNAP) is a multisubunit enzyme composed of a catalytically active core (β′βα2ω); subunits that are evolutionarily related to β′, β, α, and ω are present in DNA-dependent RNAPs of all organisms. The bacteriophage T7 Gp2 is essential for phage growth by inhibiting E.

How does the T7 expression system work?

T7 expression hosts such as DE3 prophage strains or T7 Express strains carry a chromosomal copy of the phage T7 RNA polymerase gene. When inducer is added, T7 RNA polymerase is expressed and becomes dedicated to target gene transcription. The lysY gene product lacks amidase (lysozyme) activity against the E.

What is the origin of T7 polymerase?

Bacteriophage T7 DNA replication is initiated at a site 15% of the distance from the genetic left end of the chromosome. This primary origin contains two tandem T7 RNA polymerase promoters (phi 1.1A and phi 1.1B) followed by an A + T-rich region. Such regulation may play a role during T7 DNA replication.

What is T7 tag?

The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system.

What is the T7 terminator sequence?

The bacteriophage T7 terminator (T7-T phi) encodes an RNA sequence that can form a stable stem-loop structure followed by a run of six uridylate residues; termination occurs at a 3′ G residue just downstream of the U run. …

Where does T7 polymerase come from?

1.2 Discovery of orthogonal T7 RNAP-T7 promoter pairs (T7 RNAP: pT7) T7 RNAP is a small (99 kDa) DNA-dependent RNAP derived from bacteriophage T7 (Chamberlin and Ring, 1973; Golomb and Chamberlin, 1974; Steitz, 2004). The polymerase efficiently and specifically transcribes genes bearing a T7 promoter (PT7).

What is the function of RNA polymerase?

RNA polymerase (green) synthesizes RNA by following a strand of DNA. RNA polymerase is an enzyme that is responsible for copying a DNA sequence into an RNA sequence, duyring the process of transcription.

What does RNA polymerase core enzyme do?

RNA polymerase (RNAP) is the essential enzyme responsible for transcribing the genetic information stored in DNA to RNA.

What does T7 do to E. coli?

T7 RNA polymerase is a very active enzyme: it synthesizes RNA at a rate several times that of E. coli RNA polymerase and it terminates transcription less frequently; in fact, its transcription can circumnavigate a plasmid, resulting in RNA several times the plasmid length in size.