What is Lucifer yellow dye used for?

What is Lucifer yellow dye used for?

The fluorescent dye Lucifer yellow (LY) was introduced in 1978, and has been extremely useful in studying cell structure and communications. This dye has been used mostly for labelling cells by intracellular injection from microelectrodes.

Why is it called Lucifer Yellow?

Lucifer yellow is a fluorescent dye used in cell biology. The key property of Lucifer yellow is that it can be readily visualized in both living and fixed cells using a fluorescence microscope. Lucifer yellow was invented by Walter W….Lucifer yellow.

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What is fluorescein sodium used for?

Fluorescein sodium, the sodium salt of fluorescein, is used extensively as a diagnostic tool in the field of ophthalmology and optometry, where topical fluorescein is used in the diagnosis of corneal abrasions, corneal ulcers and herpetic corneal infections.

What is Luciferian potassium?

Lucifer Yellow CH potassium salt (LY CH potassium salt) is a widely used polar molecular tracer for studying neuronal morphology. The fluorescent molecule contains a carbohydrazide that allow the molecule to be aldehyde-fixable.

Is fluorescein acidic or basic?

At pH values from 2 to 4, fluorescein exists in its neutral species, and as the pH values become more basic, within the range of 4.3 to 6.4, the monoanionic form is present.

What is fluorescein made out of?

Fluorescein is an organic compound that has wide use as a synthetic coloring agent. It is prepared by heating phthalic anhydride and resorcinol over a zinc catalyst, and it crystallizes as a deep red powder.

Does fluorescein glow in the dark?

Fluorescein, as the name implies, is a chemical that will exhibit fluorescence. In this demonstration, a small sample of fluorescein is diluted in water, then added to a cuvette. When held under a blacklight (ultraviolet radiation source) the sample will glow.

What is the pH of fluorescein?

In the pH range of existence of the fluorescein monoanion (at about pH 5.5), the fluorescence spectrum isessentially the same as that of the neutral molecule (with a slight contribution of dianion emission).

Why is fluorescein used?

Fluorescein is a diagnostic contrast agent particularly used in various ophthalmic procedures, such as checking for any corneal or vessel abnormalities. The application of fluorescein also extends to bioimaging of whole anatomic structures and even further to cellular components in immunohistological staining.

Is fluorescein toxic?

Fluoresceinating sodium salt (which will be referred to as “fluorescein”) is a non-toxic dye that produces an intense green fluorescence colour when dissolved in water. It has been safely used to detect ophthalmic lesions including ulcers and degeneration of the cornea in humans [4], [5], [6], [7].

What does fluorescein stain in the eye?

This is a test that uses orange dye (fluorescein) and a blue light to detect foreign bodies in the eye. This test can also detect damage to the cornea. The cornea is the outer surface of the eye.

What is Lucifer yellow ch?

Lucifer yellow CH, lithium salt is a water-soluble dye with excitation/emission peaks of 428/536 nm.

Is the Lucifer yellow ch monolayer suitable for flavonoid transport?

The permeability of Lucifer yellow CH is also consistent with that reported in a previous study [16]. Our results indicate that the Caco-2 monolayer in this study is well suited to represent flavonoid transport and the data are valid.

What is the solubility of lithium in Lucifer yellow ch?

The lithium salt form of the lucifer yellow CH is commonly used for microinjection because it has higher solubility than the potassium and ammonium salt forms of lucifer yellow CH. For Research Use Only. Not for use in diagnostic procedures. Store at room temperature and protect from light.

What is L453 dye used for?

Catalog number: L453. Description Lucifer yellow CH, lithium salt is a water-soluble dye with excitation/emission peaks of 428/536 nm. It is a favorite tool for studying neuronal morphology, because it contains a carbohydrazide (CH) group that allows it to be covalently linked to surrounding biomolecules during aldehyde fixation.