What is Cumate used for?

Since cumate is the effector molecule that regulates the CymR-mediated expression and therefore CymR-DNA binding, it is the molecule we have used to regulate expression from the mammalian expression system incorporating CymR.

What is a Cumate?

Cumate, a non-toxic, small-molecule inducer. CymR, a repressor that binds to cumate operator sequences in the absence of cumate. SparQ Lentivector that contains an MCS to clone-in your gene-of-interest, the cumate inducible promoter with cumate operator sequences (CuO) upstream of the MCS, and one or more markers.

What is inducible gene expression?

The production of new proteins in response to external stimuli results largely from rapid activation of gene transcription — this is known as inducible gene expression. Inducible genes are highly regulated and must be able to be rapidly and specifically activated in response to stimuli.

What is inducible system?

Inducible expression systems are essential molecular tools for production of recombinant proteins in cells, for synthesis and degradation of small molecules catalyzed by the enzymes expressed from the expression system, and for testing the function of unknown genes or proteins in cells.

What is a Repressible gene?

Definition. Repressible genes are those in which the presence of a substance (a co-repressor) in the environment turns off the expression of those genes (structural genes) involved in the metabolism of that substance.

Are constitutive genes regulated?

Constitutive genes are always being expressed (usually at a basal/regular level) but regulated genes are only expressed under certain necessary conditions in order to save cellular energy. This is an advantage because it means the bacteria only needs one operon in order to regulated multiple genes.

What are constitutive genes?

Constitutive genes are those that are always active. Genes for ribosomes are an example. They are constantly being transcribed because ribosomes are constantly needed for protein synthesis. For example, some genes needed for nerve cell function will be silenced in muscle cells.

What is an inducible system?

What are the two stages of gene expression?

It consists of two major steps: transcription and translation. Together, transcription and translation are known as gene expression. During the process of transcription, the information stored in a gene’s DNA is passed to a similar molecule called RNA (ribonucleic acid) in the cell nucleus.

How is Allolactose formed?

When water is the acceptor, hydrolysis occurs (k3), and Gal is produced. When Glc is the acceptor, allolactose is formed (k4). Most, if not all, β-galactosidases (including lacZ β-galactosidase) catalyze intermolecular allolactose synthesis. Glc simply competes with water to form allolactose (16).

What is an inducible promoter?

One of the most commonly used prokaryotic promoters is the negative inducible pLac promoter. This promoter requires removal of the lac repressor (lacI protein) for transcription to be activated. When arabinose is absent, regulatory protein AraC binds O and I1 sites upstream of pBad, blocking transcription.

What are the 3 types of gene regulation?

All three domains of life use positive regulation (turning on gene expression), negative regulation (turning off gene expression), and co-regulation (turning multiple genes on or off together) to control gene expression, but there are some differences in the specifics of how these jobs are carried out between …