What does cpDNA stand for?
Chloroplast DNA (cpDNA) is the DNA present in the organelle chloroplast. It is sometimes called the plastosome to refer to the genome of the chloroplasts as well as other plastids. It is one of the extranuclear DNA in eukaryotes.
What are the chloroplast markers?
Chloroplast markers are effective barcodes in discriminating many plant species, such as Myristicaceae (Newmaster et al., 2008), Pedicularis (Yu et al., 2011), Asteraceae (Gao et al., 2010), Carex and Kobresia (Clerc-Blain et al., 2010).
Why is rbcL gene used in dna barcoding?
The rbcL gene is a part of DNA sequence located in cpDNA and has a chance to be used as a DNA barcode [14–17] because this coding region is given universality and ease in amplifying and analyzing . This gene provides many characters to study phylogenetic because it has full length approximately 1400 bp .
Is chloroplast and Chloroplastid the same?
In biology, a chloroplast refers to the organelle found within the cell of plants and other photosynthetic eukaryotes that is filled with the green pigment called chlorophyll. Etymology: from Greek “chloros”, meaning “green” and “plast”, meaning “form” or “entity”. Synonyms: chloroplastid; green plastid; chloroleucite.
Is chloroplast contain DNA?
Each chloroplast contains a single DNA molecule present in multiple copies. The number of copies varies between species; however, the pea chloroplasts from mature leaves normally contain about 14 copies of the genome. There can be in excess of 200 copies of the genome per chloroplast in very young leaves.
Is chloroplast double membrane?
Plant chloroplasts are large organelles (5 to 10 μm long) that, like mitochondria, are bounded by a double membrane called the chloroplast envelope (Figure 10.13). In addition to the inner and outer membranes of the envelope, chloroplasts have a third internal membrane system, called the thylakoid membrane.
Where is rbcL gene found?
RbcL gene is located in the large single copy region of chloroplast genome [43, 44].
Does Cpdna have introns?
When scientists use viral enzymes to make cDNA from RNA isolated from the cells and tissues that they are studying, it does not contain introns due to being spliced out in mRNA. cDNA also does not contain any other gDNA that does not directly code for a protein (referred to as non coding DNA).