What are the markers for dendritic cells?

Overview of human dendritic cell lineages In humans, all DCs express high levels of MHC class II (HLA-DR) and lack typical lineage markers CD3 (T cell), CD19/20 (B cell) and CD56 (natural killer cell).

Do dendritic cells express CD11b?

CD11b is considered a pan-myeloid marker (expressed after granulocyte-monocyte progenitors (GMP) phase in the bone marrow). CD11c is a prefered marker for dendritic cells. In general, mouse DCs express CD11c but are negative for CD11b.

How do you identify a dendritic cell?

Dendritic cells (DC) are bone marrow-derived immune cells that can be found in the blood, lymphoid organs, and various tissues in the body. They are generally stellate in morphology, with branching dendrites that resemble those of neurons.

Do macrophages express CD83?

In the present study, we found that CD83 is constitutively expressed inside monocytes and macrophages, as well as in imDCs. Upon activation of the cells with LPS, CD83 was also rapidly induced on the surface of these cells.

What is the difference between pDC and cDC?

A series of studies have outlined distinct differences in the ability of DC subsets to induce immune responses. In particular, cDC have been implicated in driving a pro-allergic response, while pDC have been identified to block or “tolerize” the pulmonary immune environment against Th2 responses [6].

What is CD14 a marker for?

Cluster of differentiation 14 (CD14) was described as monocyte/ macrophage differentiation antigen on the surface of myeloid lineage, such as monocytes, macrophages and dendritic cells (DCs). It has been commonly used in normal tissue or blood and in leukemia as a marker for myeloid cells.

What is CD11b a marker for?

CD11b expression as a marker to distinguish between recently activated effector CD8+ T cells and memory cells.

What is dendritic cell?

Listen to pronunciation. (den-DRIH-tik sel) A special type of immune cell that is found in tissues, such as the skin, and boosts immune responses by showing antigens on its surface to other cells of the immune system. A dendritic cell is a type of phagocyte and a type of antigen-presenting cell (APC).

Where is CD40 expressed?

CD40 is constitutively expressed by antigen presenting cells, including dendritic cells, B cells and macrophages. It can also be expressed by endothelial cells, smooth muscle cells, fibroblasts and epithelial cells.

What are CD11c cells?

CD11c, also known as integrin alpha X, is the most widely used defining marker for dendritic cells (DCs). CD11c can bind complement iC3b and mediate phagocytosis in vitro, for which it is also referred to as complement receptor 4.

Are PDC myeloid cells?

Plasmacytoid dendritic cells (pDCs) can be derived from both myeloid and lymphoid progenitors.

Are plasmacytoid dendritic cells antigen presentation?

One of the remaining enigmas of the dendritic cell (DC) network is the potential contribution of plasmacytoid DCs (pDCs) to antigen presentation. Instead, pDCs are regarded as immunomodulating cells, capable of directing the immune response through their secretion of large amounts of type I interferons.

What is the role of CD83 on dendritic cells?

Human CD83 is a marker molecule for mature dendritic cells (DC) and is also expressed on activated B and T cells. Although CD83 has been implicated in immune responses, its function on DC and T cells remains unclear. In this study, we wanted to assess the role of CD83 expressed on DC and T cells in …

What is cdcd83 and what does it do?

CD83 is one of the most characteristic cell surface markers for fully matured dendritic cells (DCs). In their function as antigen presenting cells they induce T-cell mediated immune responses. In this review we provide an overview on well described and proposed functions of this molecule as well as …

What is intracellular preformed CD83?

Intracellular preformed CD83 protein is detected in a wide range of immune cells, including immature DC, monocytes, macrophages, natural-killer (NK) cells and lymphocytes ( 35 ). CD83 is rapidly transported to the surface from golgi and recycling endosome pools in DC, macrophages, monocytes and B-cells upon TLR or TNF engagement ( 37, 47 ).

Is cdcd83 a marker of B-cell activation?

CD83 expression is a sensitive marker of activation required for B-cell and CD4+T-cell longevity in vivo. J Immunol. (2007) 179:4550–62. 10.4049/jimmunol.179.7.4550 [PubMed] [CrossRef] [Google Scholar] 67.