Is UltraPure water nuclease free?

Nuclease-free water is free of both DNAse and RNAse, and involves treating with DEPC (diethylpyrocarbonate) and/or autoclaving to inactivate RNAse and DNAse. Ultrapure water, on the other hand, is obtained by ultrafiltration to achieve the high level of purity. It also does not contain any nucleases.

What is nuclease-free water?

Nuclease-Free Water is pure, quality-tested water suitable for use in all experiments that require nuclease-free water, including molecular biology applications. Nuclease-Free Water is provided in nuclease-free containers. Nuclease-Free Water is no longer available in a 5 liter bag with luer cap (cat.

Is UltraPure water DNase free?

UltraPure™ DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. It is 0.1-µm membrane-filtered and tested for DNase and RNase activity.

What is the difference between deionized water and UltraPure water?

Ultrapure water (UPW), formulated as H2O, contains no contaminants. This deionized quality of water has no assurance on the contaminant levels unlike in the case of ultrapure water, where the purity is 100%. …

Is nuclease free water RNase free?

Nuclease-Free Water is used in various molecular biology applications requiring nuclease-free conditions, such as in processing DNA or RNA (i.e. PCR, cDNA synthesis, or qPCR). This product is sterile-filtered (0.2 µm), free of RNase and DNase activity, endotoxin-free, and not DEPC-treated.

Is nuclease free water distilled?

UltraPure DNAse/RNase-Free Distilled Water.

Is nuclease free water a PCR grade?

Description. Nuclease-free water is an essential component of all PCR-based molecular biology work. This PCR grade water is highly purified, DEPC treated and autoclaved to remove RNAses and DNAses, and is ideal for routine PCR work.

Is ultrapure water sterile?

UltraPure Sterile Water consists of highly polished pharmaceutical grade deionized water processed using sterile conditions This product was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers.

Is Milli-Q water nuclease free?

Since the cartridge is designed to be connected to the outlet of a water purification system, such as a Milli-Q®, one can obtain ultrapure water that is also free of nucleases.

What is the difference between pure and ultrapure water?

Ultrapure water is water that has been made as close as possible to pure H2O. The electric resistivity ratio of theoretically pure water is 18.24 MΩcm, and ultrapure water has been made as close as possible to this level. In fact, there is also no precise definition for ultrapure water.

What are the risks of using nuclease free water?

Contamination by unwanted DNA is often more of a hazard than nuclease contamination (these are typically denatured by the high temperatures used in PCR). If you want to exercise an abundance of caution, use nuclease free water.

Is DNase free water the same as nuclease free water?

“DNase-free, RNase-free water” is the same thing as “nuclease-free” water since DNase and RNase are the exact “nucleases” in question. Whether you trust the company or not is most likely the concern.

What kind of nuclease-free water do you offer?

We offer several brands and molecular grades of nuclease-free water. To meet the special challenges of working with RNA, our Invitrogen waters are certified nuclease-free.

What is Invitrogen nuclease free water?

Invitrogen Nuclease-Free Water Not DEPC-treated Autoclaved, membrane-filtered RNase and DNase-free Ideal for applications that may be acutely sensitive to residual DEPC (i.e., oocyte injection)