Is Foxp3 a surface protein?

No. 88-8996-40). Cells in the lymphocyte gate were used for analysis. Foxp3 protein is difficult to stain since it is not very abundant, and it is an intracellular protein.

What cells express Foxp3?

It has been considered initially as the only specific marker for Treg. However, recent work has proposed that Foxp3 can be expressed by other types of lymphoid cells or myeloid cells and also by some non-hematopoietic cells such as epithelial cells.

What are Foxp3 Tregs?

FOXP3+ regulatory T (Treg) cells are critical in maintaining immune tolerance and homeostasis of the immune system. The molecular mechanisms underlying the stability, plasticity and functional activity of Treg cells have been much studied in recent years.

Is FOXP3 a surface marker?

FOXP3 is a 50-55 kD transcription factor, also known as Forkhead box protein P3, Scurfin, JM2, or IPEX. It is proposed to be a master regulatory gene and more specific marker of T regulatory cells than most cell surface markers.

Is FOXP3 a transcription factor?

The protein encoded by FOXP3, termed scurfin, is a transcription factor that plays a critical role in the development and function of CD4+CD25+ Tregs (Chapter 18). Affected patients suffer from severe autoimmunity in infancy.

What is the role of FOXP3?

The FOXP3 gene provides instructions for producing the forkhead box P3 (FOXP3) protein. The FOXP3 protein attaches (binds) to specific regions of DNA and helps control the activity of genes that are involved in regulating the immune system.

What do Tregs do?

Regulatory T cells (Tregs) are a specialized subpopulation of T cells that act to suppress immune response, thereby maintaining homeostasis and self-tolerance. It has been shown that Tregs are able to inhibit T cell proliferation and cytokine production and play a critical role in preventing autoimmunity.

How do you stain FOXP3?

FOXP3 Intracellular Staining Procedures: Add 1 ml of 1X BioLegend’s FOXP3 Fix/Perm solution to each tube, vortex and incubate at room temperature in the dark for 20 minutes, then spin down the cells and remove the supernatant. 3. Wash once with cell staining buffer (Cat.

Do all Tregs express CD25?

Unfortunately, accumulating evidence suggests that the above-listed markers are not strictly Treg-specific. Upon activation, all T cells express CD25, the α-chain of the interleukin-2 (IL-2) receptor [51–53], IL-2 being a T-cell growth factor which is important for T-cell clonal expansion.

What is the function of FOXP3?

What is FOXP3 transcription factor?

Foxp3 is the major transcription factor controlling T-regulatory cells (Treg or CD4+ cells). CD4+ cells are leukocytes responsible for protecting animals from foreign invaders such as bacteria and viruses.

What is Invitrogen mouse IL-6 Elisa?

The Invitrogen™ Mouse IL-6 ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA). This assay is designed to detect and quantify the level of mouse IL-6 in mouse serum, plasma, buffered solution, or cell culture medium. The assay recognizes both natural and recombinant mouse IL-6.

What is the IL-6 Ready-Set-Go ELISA kit?

The eBioscience IL-6 Ready-SET-Go! ELISA kit offers an easy and sensitive method to quantify IL-6 levels in biological samples.

What kind of ELISA kit do you use for mice?

RayBio® Mouse IL-6 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples. Password Forgot your password? Need more sensitivity? Check out the new IQELISA ™ kit for this target.

Is this ELISA kit any good?

I have used this Elisa kit, its quite affordable considering the amount of time saved and data generated from it. I have even compared IL-6 ELISA data to IL-6 luminex data (from the same samples) and they are relatively similar. I used this kit with LPS-inflammed RAW264.7 cells supernatants.