How does methylation microarray work?

This work is dedicated to the development of a technology for unbiased, high-throughput DNA methylation profiling of large genomic regions. In this method, unmethylated and methylated DNA fractions are enriched using a series of treatments with methylation sensitive restriction enzymes, and interrogated on microarrays.

How do you test for DNA methylation?

Currently, there are three primary methods to identify and quantify DNA methylation. These are: sodium bisulfite conversion and sequencing, differential enzymatic cleavage of DNA, and affinity capture of methylated DNA (1). Restriction enzyme based differential cleavage of methylated DNA is locus-specific.

What is DNA methylation analysis?

Methylation analysis is the study of chromosomal patterns of DNA or histone modification by methyl groups. Methylation constitutes an epigenetic mark because its location can affect the regulation of gene expression in a heritable fashion.

Can DNA sequencing detect methylation?

After PCR amplification, uracil residues are converted to thymine. DNA methylation status can be determined by direct PCR sequencing or cloning sequencing.

What is Methylation array analysis?

Introduction to Methylation Array Analysis DNA methylation plays an important and dynamic role in regulating gene expression. Methylation arrays enable quantitative interrogation of selected methylation sites across the genome, offering high-throughput capabilities that minimize the cost per sample.

Does microarray test for Prader Willi?

An array is especially recommended when the diagnosis of PW or AS is not certain, or in very young paediatric cases. Microarray testing will detect ~70% of PWS or AS, ie. all cases caused by chromosome 15 deletion and some cases caused by UPD(15).

What is 450K Array?

Illumina Infinium HumanMethylation450 (450K) BeadChip array, which covers over 480K CpG sites and targets 96% of CpG islands in human genome [5], has been widely utilized in many large studies, such as The Cancer Genome Atlas (TCGA) and The International Cancer Genome Consortium (ICGC) Project [6].

What does a DNA microarray test for?

The DNA microarray is a tool used to determine whether the DNA from a particular individual contains a mutation in genes.

Are DNA methylation microarrays accurate?

Even though DNA methylation microarrays analyze a small fraction of 28 million CpG sites present in the human genome, they offer cost-effective and consistent analysis of many biologically relevant genomic regions. This chapter describes an accurate, scalable, and flexible microarray technology for DNA methylation profiling.

Is there a high-throughput technology for DNA methylation profiling?

This work is dedicated to the development of a technology for unbiased, high-throughput DNA methylation profiling of large genomic regions. In this method, unmethylated and methylated DNA fractions are enriched using a series of treatments with methylation sensitive restriction enzymes, and interrogated on microarrays.

What is Illumina DNA methylation array?

Illumina DNA methylation arrays are based on “genotyping” of bisulfite-converted genomic DNA. Treatment of a DNA sample with bisulfite results in a change in sequence from C to U for unmethylated cystosines, whereas methylated cytosines remain unchanged.

What are the different types of microarray-based assays?

The microarray-based approaches are largely based on three major techniques: restriction enzyme digest, sodium bisulfite conversion of genomic DNA, and affinity-based assays.