How do you give an intraperitoneal injection?

How do you give an intraperitoneal injection?

For intraperitoneal administration, restrain the mouse with one hand and tilt the head backward to expose the abdomen. Insert the needle at a shallow angle through the musculature in the lower left abdomen. Push the needle 2–3 mm further to avoid leakage at the injection site.

Where do you ip injection mice?

On the mouse you want to make your IP injection in the lower right or left quadrant of abdomen trying to avoid hitting bladder, liver, or other internal organs.

What is the maximum volume for intraperitoneal injection mouse?

The recommended maximum volume for administration is 1% of body weight (e.g., a 20 gm mouse can be given 0.2 ml).

What is the intraperitoneal route?

Intraperitoneal injection or IP injection is the injection of a substance into the peritoneum (body cavity). It is more often applied to animals than to humans. In humans, the method is widely used to administer chemotherapy drugs to treat some cancers, particularly ovarian cancer.

How many ml can be given subcutaneously?

Medications administered by subcutaneous injection include drugs that can be given in small volumes (usually less than 1 mL but up to 2 mL is safe). Insulin and some hormones are commonly administered as subcutaneous injections.

What is intraperitoneal injection used for?

In humans, the method is widely used to administer chemotherapy drugs to treat some cancers, particularly ovarian cancer. Although controversial, intraperitoneal use in ovarian cancer has been recommended as a standard of care. Fluids are injected intraperitoneally in infants, also used for peritoneal dialysis.

What drugs are given intraperitoneal?

Drugs most commonly applied for intraperitoneal chemotherapy include mitomycin C, cisplatin, carboplatin, oxaliplatin, irinotecan, 5-fluoruracil, gemcitabine, paclitaxel, docetaxel, doxorubicin, premetrexed and melphalan. The drugs and their doses that are used vary widely among centres.