How do you draw blood from catfish?

This procedure targets the dorsal aorta (a relatively larger blood vessel) in a sedated fish, punctured by inserting a needle directly from the anterior part of the anal fin about 2-5 mm behind the genital papilla, to draw the desired amount of blood.

How do animals get blood samples?

The animal is restrained manually or using a suitable animal restrainer. Hind leg is immobilized and slight pressure may be applied gently above the knee joint. The vein is punctured using a 20G needle and enough volume of blood is collected with a capillary tube or a syringe with a needle.

Where do you draw blood from a fish?

Cardiac Puncture: Blood is collected from the heart ventricle. Insert needle perpendicular to the ventral surface of the fish in the centre of an imaginary line between the anterior most part of the base of the pectoral fins.

What color is fish blood?

The blue-green coloration of the blood plasma in some marine fishes, which is attributed to a protein bound tetrapyrrol (biliverdin), is an anomaly in vertebrates.

Why would a blood sample be taken from an animal?

Taking a blood sample is one of the most common procedures performed on laboratory animals, used for example: For analysis of biochemical, metabolic, toxicological or immunological parameters. For examination or culture of micro-organisms. For production of antibodies.

Which are the methods of collection of blood?

Three popular methods of blood collection are:

• Arterial Sampling.
• Venipuncture Sampling.
• Fingerstick Sampling.

How do you take a blood sample from a fish?

A blood sample can be drawn from either an anesthetized or freshly-euthanized fish. Remove the fish from the holding tank and hold it upside down. Have an assistant pick up the fish in both hands and hold it upside down for you. Keep the body of the fish straight and the tail facing towards you.

What is a good sample size to take from a fish?

A good sample size to safely take from a fish is 1 mL of blood. Remove the needle from the syringe and throw it away in a safe disposal container. Take off the needle and place it in a sharps container or biohazard disposal container.

How do you draw blood from a fish with insulin?

You can use an insulin syringe with a 22-gauge needle to withdraw blood from a fish without causing the fish harm. Push the needle toward the vertebral column until it reaches the base. Gently slide the needle in until you feel it hit the base of the fishes spinal column.

What is fish used for in medical laboratory science?

Medical center lab personnel and oncologists use FISH to help assess patients who may have cancer, and sometimes to monitor a patient who has already been diagnosed with cancer and treated. 2